A Comparative Invitro Study Investigating the Dentinal Changes Caused by Commercially Available Desensitizing Toothpastes and Laser - Original Research Article
DOI:
https://doi.org/10.12974/2311-8695.2015.03.01.1Keywords:
Dentinal hypersensitivity, EDX, Helium-neon laser, SEM, Tubular blocking.Abstract
Aim: The aim of this study was to compare the level of dentinal tubule blocking and mineralization achieved by the use of two commercially available dentifrices (Vantej® and Colgate® sensitive Pro-relief) with or without the use of laser for the management of dentinal hypersensitivity.
Method: Sixty extracted human molar teeth were used for this study. The teeth were sectioned into dentin discs of 2-4mm thickness. These specimens were then randomly divided into 6 groups and were treated with Vantej® tooth paste, Colgate Sensitive Pro-relief® tooth paste, Helium- Neon (He-Ne) laser, combination of He-Ne laser and Vantej® tooth paste and a combination of He-Ne laser and Colgate Sensitive Prorelief® tooth paste. The specimens were then analyzed under scanning electron microscope (SEM) for the evaluation of dentinal tubular blocking. The elemental analysis of the tubular content was evaluated using Energy Dispersive Xray Spectroscopy (EDX).
Results: The SEM analysis showed significant difference in the mean percentage scores within the individual groups. The Laser with Colgate Sensitive Prorelief® tooth paste group showed complete blocking of the dentinal tubules which was statistically significant (P<0.001), similar results were also observed with the Pro-relief® alone toothpaste group. The Vantej® tooth paste group also exhibited significant tubular blocking. The Laser alone group and combination of laser and Vantej® tooth paste group showed only partial tubular blocking. EDX analysis of the experimental groups revealed peaks of the elemental contents found in the experimental specimens.
Conclusion: The data obtained from this study demonstrated that the Colgate sensitive Pro-relief® toothpaste alone and /or when used in combination with laser therapy was found to most effective in the blocking of dentinal tubules.
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