Preliminary Analysis of Phenolic Acid Composition of Phlomis syriaca

Abstract

Phlomis genus is one of the genera of Lamiacea family with various pharmaceutical activity. Although phythochemical constituents and pharmacological activities of most of the Phlomis genus are well documented, there is a scarce of research on Phlomis syriaca. This study was performed to reveal phenolic acids of P. syriaca inorder to encourage more studies based on its phytochemicals to elucidate the pharmacalogical activity of this plant. Total phenolic compound amount of P. syriaca was detected as 48.46 ± 4.30 μg GAEs/mg extract according to Folin– Ciocalteu method. An HPLC method was developed to quantify the amounts of nine phenolic compounds in the methanol extract of P.syriaca. The developed analytical system led to the separation, identification and the quantification of nine phenolic compounds most frequently found in plants belonging to the Lamiaceae family. The phenolic acids with determined concentrations in descending order were as follows, respectively; p-coumaric acid (5.334 mg/L), caffeic acid (2.367 mg/L), 4-hydroxybenzoic acid (1.978 mg/L), ferulic acid (1. 052 mg/L), chlorogenic acid (0.581 mg/L), rosmarinic acid (0.546 mg/L), protocautechic acid (0.287 mg/L), gallic acid (0.186 mg/L). cinamic acid (0.064 mg/L).