A Validated Quantitative HPLC Method for Proanthocyanidin A2 in Pometia pinnata Leaves
DOI:
https://doi.org/10.12974/2311-858X.2017.05.01.2Keywords:
Validation, microwave, extraction, accuracy, precision.Abstract
A reverse-phase high-performance liquid chromatographic method was described for the determination of proanthocyanidin A2 in Pometia pinnata leaf extracts. The method utilized a Phenominex® Luna 5u Hilic column with a mixture of 2% acetic acid and acetonitrile (step gradient elution as follows: 0-4 min, 5:95; 5-9 min, 10:90; 10-14 min, 80:20 v/v) as the mobile phase at a flow rate of 1 mL/min, and UV detection at 280 nm. The parameters of linearity, precision, accuracy, specificity and sensitivity of the method were evaluated. The extraction methods for proanthocyanidin A2 were also examined. Proanthocyanidin A2 was eluted within 7 min with a satisfactory peak resolution. The recovery of the HPLC method was 96-98% with a good linearity (r2≥ 0.9999) for proanthocyanidin A2 in the concentration range of 7.7 - 250 µg/mL. A high degree of specificity as well as repeatability and reproducibility (RSD values less than 5%) were also achieved. The limits of detection and quantification were 1.25 and 2.50 µg/mL, respectively. This eatablished specific, precise, accurate, rapid and reproducible HPLC method was successfully used to quantify the active principle, proanthocyanidin A2 in P. pinnata leaf extracts. Proanthocyanidin A2 was found to be a major constituent in the crude methanol extract of P. pinnata leaves, at 33.8 ± 0.4 mg/g dried extract. Microwave-assisted extraction (MAE) was selected as the best extraction method for proanthocyanidin A2. The optimized MAE method increased the amount of proanthocyanidin A2 extracted from the dried leaf powder up to 36.6 %w/w.
References
Wiart C. Medicinal plants of Asia and the Pacific. New York: CRC Press Taylor & Francis Group 2006.
Ali M. Medicinal plant diversity and vegetation analysis of logged over hill forest of Tekai Tembeling Forest Reserve, Jerantut, Pahang. J Agric Sci 2010; 2: 189-210.
Suprapta DN, Suwari IGANA, Arya N, Ohsawa K. Pometia pinnata leaves extract to control late blight disease in potato. ISSAAS 2002; 8: 31-6.
Suedee A, Tewtrakul S, Panichayupakaranant P. Anti-HIV-1 integrase compound from Pometia pinnata leaves. Pharm Biol 2013; 51: 1256-61. https://doi.org/10.3109/13880209.2013.786098
Gallina L, Pozzo FD, Galligioni V, Bombardelli E, Scagliarini A. Inhibition of viral RNA synthesis in canine distemper virus infection by proanthocyanidin A2. Antiviral Res 2011; 92: 447-52. https://doi.org/10.1016/j.antiviral.2011.10.004
Xu X, Xie H, Wang Y, Wei X. A-Type proanthocyanidins from lychee seeds and their antioxidant and antiviral activities. J Agric Food Chem 2010; 58, 11667-72. https://doi.org/10.1021/jf1033202
Iwasawa A, Niwano Y, Mokudai T, Kohno M. Antiviral activity of proanthocyanidin against feline calicivirus used as a surrogate for noroviruses, and coxsackievirus used as a representative enteric virus. Biocontrol Sci 2009; 14, 107-11. https://doi.org/10.4265/bio.14.107
Brinkworth RI, Stoermer MJ, Fairlie DP. Flavones are inhibitors of HIV-1 proteinase. Biochem Bioph Res Commun 1992; 188: 631-7. https://doi.org/10.1016/0006-291X(92)91103-W
Shahat AA, Ismail SI, Hammouda FM, Azzam SA, Lemière G, Bruyne T, et al. Anti-HIV activity of flavonoids and proanthocyanidins from Crataegus sinaica. Phytomedicine 1998; 5: 133-6. https://doi.org/10.1016/S0944-7113(98)80010-X
Baiges I, Palmfeldt J, Blade´ C, Gregersen N, Arola L. Lipogenesis is decreased by grape seed proanthocyanidins according to liver proteomics of rats fed a high fat diet. Mol Cell Proteomics 2010; 9: 1499-513. https://doi.org/10.1074/mcp.M000055-MCP201
Hsieh MC, Shen YJ, Kuo YH, Hwang LS. Antioxidative activity and active components of Longan (Dimocarpus longan Lour.) flower extracts. J Agric Food Chem 2008; 56: 7010-6. https://doi.org/10.1021/jf801155j
Koerner JL, Hsu VL, Lee J, Kennedy JA. Determination of proanthocyanidin A2 content in phenolic polymer isolates by reversed-phase high-performance liquid chromatography. J Chromatogr A 2009; 1216: 1403-9. https://doi.org/10.1016/j.chroma.2008.12.086
ICH. Guideline Q2(R1)-validation of analytical procedures: Text and methodology. Geneva: ICH 2005.
Hayat K, Hussain S, Abbas S, Farooq U, Ding B, Xia S, et al. Optimized microwave-assisted extraction of phenolic acids from citrus mandarin peels and evaluation of antioxidant activity in vitro. Sep Purif Technol 2009; 70: 63-70. https://doi.org/10.1016/j.seppur.2009.08.012
Yan MM, Liu W, Fu YJ, Zu YG, Chen CY, Luo M. Optimisation of the microwave-assisted extraction process for four main astragalosides in Radix Astragali. Food Chem 2010; 119: 1663-70. https://doi.org/10.1016/j.foodchem.2009.09.021
Proestos C, Komaitis M. Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds. Food Sci Technol 2008; 41: 652-59. https://doi.org/10.1016/j.lwt.2007.04.013
Zhang B, Yang R, Liu CZ. Microwave-assisted extraction of chlorogenic acid from flower buds of Lonicera japonica Thunb. Sep Purif Technol 2008; 62: 480-3. https://doi.org/10.1016/j.seppur.2008.02.013
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Copyright (c) 2017 Areerat Suedee, Supinya Tewtrakul, Pharkphoom Panichayupakaranant
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