Effects of Lead Stress on Growth and Some Physiological Characteristics of Bean

Authors

  • Raziye Kul Ataturk University, Faculty of Agriculture, Department of Horticulture, Erzurum-Turkey
  • Melek Ekinci Ataturk University, Faculty of Agriculture, Department of Horticulture, Erzurum-Turkey
  • Ertan Yildirim Ataturk University, Faculty of Agriculture, Department of Horticulture, Erzurum-Turkey

DOI:

https://doi.org/10.12974/2311-858X.2019.07.3

Keywords:

Phaseolus vulgaris, Heavy metal, Physiology.

Abstract

This study was conducted to determine the effects of different lead (0, 1000, 1500 and 2000 mg/kg) levels on physiological and morphological responses of bean plants in 2019 in Atatürk University, Faculty of Agriculture, Department of Horticulture under laboratory and greenhouse conditions. Bean (Phaseolus vulgaris L.) Gina cultivar was used as plant material in the experiment. According to study findings, it was determined that differences between treatments were statistically important. The bean plants grown under heavy metal stress conditions were affected regarding to plant growth parameters (fresh and dry weight ect.), some plant physiological parameters such as tissue electrical conductivity (TEC), tissue rational water content (TRWC). Lead stress conditions negatively affected plant growth. Plants grown under heavy metal stress had more TEC and less TRWC values compared to the control plants. Heavy metal stress caused the decreased chlorophyll a, chlorophyll b and caretenoid content in bean plants. In conclusion, bean plants responded to lead stress by reducing growth, TRWC and pigment concentration, increasing TEC. 

References

Stresty TVS, Madhava Rao KV. Ultrastructural alterations in response to zinc and nickel stress in the root cell of pigeonpea. Environ Exp Bot 1999; 41: 3-13. https://doi.org/10.1016/S0098-8472(98)00034-3

Munzuroğlu, O, Gur, N. The effects of heavy metals on the pollen germination and pollen tube growth of apples (Malus sylvestris Miller cv. Golden). Turk J Biol 24 2000; 677-684.

Foyer CH, Lopez-Delgado H, Dat JF, Scott IM. Hydrogen peroxide and glutathione associated mechanisms of acclimatory stress and tolerance and signaling. Physiol Plant 1997; 100:241-254. https://doi.org/10.1111/j.1399-3054.1997.tb04780.x

Lombardi L and Sebastiani L. Copper toxicity in prunuscerasifera: growth and antioxidant enzymes responses of in vitro grown plants. Plant Science 2005; 168, 797-802. https://doi.org/10.1016/j.plantsci.2004.10.012

Şalk A, Arin L, Deveci M and Polat S. Özel sebzecilik (In Turkish). Namık Kemal Üniversitesi Ziraat Fakültesi Bahçe Bitkileri BoÅNlümü, Tekirdağ, Turkey 2008; 116-119.

Kaya C, Ak, BE, Higss, D, 2003. Response of salt-stressed strawberry plants to supplementary calcium nitrate and/or potassium nitrate. Journal of Plant Nutrition, 26: 543-560. https://doi.org/10.1081/PLN-120017664

Ni Z, Kim E, Chen Z. Chlorophyll and starch assays. Protocol Exchange 2009; 10: 1038. https://doi.org/10.1038/nprot.2009.12

Rodriguez-Amaya DB, Kimura M. HarvestPlus handbook for carotenoids analysis (first ed.), IFPRI and CIAT, Washington, DC and Cali (Chapter 2), 2004; 58 pp.

SPSS Inc. SPSS® 18.0 Base User's Guide. Prentice Hall 2010.

Shahid M, Pinelli E, Pourrut B, Silvestre J, Dumat C. Leadinduced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation. Ecotoxicol Environ Saf 2011; 74(1): 78-84. https://doi.org/10.1016/j.ecoenv.2010.08.037

Schützendübel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A. Cadmiuminduced changes in antioxidants systems, hydrogen peroxide content and differentiation in Scots pine roots. Plant Physiology 2001; (127), 887-898. https://doi.org/10.1104/pp.010318

Vitoria AP, Lea PJ, Azevedo RA. Antioxidant enzymes responses to cadmium in radish tissues. Phytochemistry 2001; (57), 701-710. https://doi.org/10.1016/S0031-9422(01)00130-3

Benavides MP, Gallego SM, Tomaro ML. Cadmium toxicity in plants. Brazilian Journal of Plant Physiology 2005; (17), 21- 34. https://doi.org/10.1590/S1677-04202005000100003

Gratao LP, Polle A, Lea P, Azevedo A. Making the life of heavy metalstressed plants a little easier. Functional Plant Biology 2005; 32, 481-494. https://doi.org/10.1071/FP05016

Iqbal N, Masood A, Nazar R, Syeed S, Khan N A. Photosynthesis, growth and antioxidant metabolism in mustard (Brassica juncea L.) cultivars differing in Cd tolerance. Agricultural Sciences in China 2010; 9, 519-527. https://doi.org/10.1016/S1671-2927(09)60125-5

Marshner P. Marschner's Mineral Nutrition of Higher Plants. (3rd Ed) Academic Press, London 2012.

Opeolu BO, Adenuga OO, Ndakidemi PA, Olujimi OO. Assessment of phyto-toxicity potential of lead on tomato (Lycopersicon esculentum L) planted on contaminated soils. Inter J Physical Sci 2010; 5(2): 68-73.

Barceló J, Poschenrieder C, Andreu I, Gunsé B. Cadmiuminduced decrease of water stress resistance in bush bean plants (Phaseolus vulgaris L. cv. Contender). I. Effects of Cd on water potential, relative water content and cell wall elasticity. J Plant Physiol 1986; 125:17-25. https://doi.org/10.1016/S0176-1617(86)80239-5

Poschenrieder C, Gunsé B, Barceló J. Influence of cadmium on water relations, stomatal resistance, and abscisic acid content in expanding bean leaves. Plant Physiol 1989; 90: 1365-1371. https://doi.org/10.1104/pp.90.4.1365

Costa G, Morel JL. Water relations, gas exchange and amino acid content in Cd-treated lettuce. Plant Physiol Biochem 1994; 32: 561-570.

Ehlert C, Maurel C, Tardieu F, Simonneau, T. Aquaporinmediated reduction in maize root hydraulic conductivity impacts cell turgor and leaf elongation even without changing transpiration. Plant Physiol 2009; 150: https://doi.org/10.1104/pp.108.131458

Manousaki E, Kalogerakis N. Phytoextraction of Pb and Cd by the Mediterranean saltbush (Atriplex halimus L.): metal uptake in relation to salinity. Environ Sc Pollut R 2009; 16: 844-854. https://doi.org/10.1007/s11356-009-0224-3 https://doi.org/10.1007/s11356-009-0224-3

Ahmad P, Nabi G, Ashraf M. Cadmium-induced oxidative damage in Mustard

[Brassica juncea L.) Czern. & Coss.] plants can be alleviated by salicylic acid. S Afr J Bot 2011; 77: 36-44. https://doi.org/10.1016/j.sajb.2010.05.003

Alyemeni MN, Ahanger MA, Wijaya L, Alam P, Ahmad P. Contrasting tolerance among soybean genotypes subjected to different levels of cadmium stress. Pak J Bot 2017; 49(3): 903-911. http://repository.psau.edu.sa:80/jspui/handle/123456789/146 7

Asri ÖF, Sönmez S. Ağır metal toksisitesinin bitki metabolizması üzerine etkileri. Batı Akdeniz Tarımsal Araştırma Enstitüsü Derim Dergisi 2006; 23 (2): 36-45.

Zengin FK, Munzuroğlu O. Effects of some heavy metals on content of chlorophyll, proline and some antioxidant chemicals in bean (Phaseolus vulgaris L.) seedlings. Acta Biol Crac Ser Bot 2005; 47/2: 157-164.

Deshna D, Bafna A. Effect of lead stress on chlorophyll content, malondialdehyde and peroxidase activity in seedlings of mung bean (Vigna radiata). Int J Res Chem Environ 2013; 3(3): 20-25.

Kasim, WA, Abokassem, EM, Ragab, GA, Sewelam, NA,. Alleviation of lead stress toxicity in Vigna unguiculata by Salicylic acid. Egypt J Exp Biol (Bot) 2014; 10(1): 37-49.

Drążkiewicz M, Baszyński T. Growth parameters and photosynthetic pigments in leaf segments of Zea mays exposed to cadmium, as related to protection mechanisms. J Plant Physiol 2005; 162(9): 1013-1021. https://doi.org/10.1016/j.jplph.2004.10.010

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Published

08-03-2019

How to Cite

Kul, R., Ekinci, M., & Yildirim, E. (2019). Effects of Lead Stress on Growth and Some Physiological Characteristics of Bean. Global Journal Of Botanical Science, 7, 15–19. https://doi.org/10.12974/2311-858X.2019.07.3

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