Foliar Gall and Antioxidant Enzyme Responses in Alstonia scholaris, R. Br. after Psylloid Herbivory– An Experimental and Statistical Analysis
DOI:
https://doi.org/10.12974/2311-858X.2014.02.01.2Keywords:
Alstonia scholaris, R. Br., Antioxidant enzymes, Chlorophyll, Foliar gall, Pauropsylla tuberculata, Crawf., Phenolics.Abstract
Alstonia scholaris, R. Br. (Apocynaceae, commonly known as Devil tree) is an elegant evergreen tree, which serves as a host for foliar galls caused by Pauropsylla tuberculata, Crawf. A. scholaris is useful both medicinally as well as being an important shade tree and gall formation being problematic for using this tree in the cities. Gall formation in mature plant does not much effect the plant but when the young plants are affected it harms the plant. Gall induction, mediated by insect-herbivore chemical stimuli, is the result of anatomical and biochemical alterations in the host-plant tissues. In order to understand the biochemical changes induced by P. tuberculata on Alstonia, we studied the chlorophyll content and secondary metabolites specially phenolics that apparently act as defense compounds and also the activity of two foliar antioxidant enzymes, catalase and peroxidase both experimentally and statistically. Activity of antioxidant enzymes and phenolics were found to be positively correlated with different stages of gall leaves whereas chlorophyll content exhibited strong negative correlation. Information from both the studies led us to suggest that antioxidant enzymes and phenolics act as biochemical markers of foliar gall resistance in Alstonia scholaris.References
Saini D, Sarin R (2012) SDS-PAGE Analysis of Leaf Galls of Alstonia scholaris (L.) R. Br. Plant Pathology and Microbiology 3:121. doi:10.4172/2157- 7471.1000121
Khare CP (2007) Indian Medicinal Plants: An Illustrated Dictionary. Berlin/Heidelberg: Springer-Verlag.
Pratyush K, Misra CS, James J, Lipin Dev MS, ThaliyilVeettil AK and Thankamani V (2011). Ethnobotanical and Pharmacological Study of Alstonia (Apocynaceae) - A Review. Journal of Pharmaceutical Sciences and Research 3: 1394-1403. http://www.jpsr.pharmainfo.in/Documents/ Volumes/Vol3Issue08/jpsr%2003110806.pdf
Chil-Woo Lee, Marina Efetova, Julia C Engelmann, Robert Kramell, Claus Wasternack, Jutta Ludwig-Müller, Rainer Hedrich, and Rosalia Deeken (2009) Agrobacterium tumefaciens Promotes Tumor Induction by Modulating Pathogen Defense in Arabidopsis thaliana
[W]. The Plant Cell 9: 2948-2962. http://dx.doi.org/10.1105/tpc.108.064576
Raman A (2007) Insect-induced plant galls of India: unresolved questions. Current Science 92: 748-757. http://www.currentscience.ac.in/Downloads/article_id_092_06 _0748_0757_0.pdf
Ananthakrishnan TN (2004) General and Applied Entomology. (2nd edn), India: Tata McGraw-Hill.
Emich F, Feigl F (1932) Microchemical Laboratory Manual. New York: Wiley and Sons. Journal of Chemical Technology and Biotechnology 51: 894-895.
Maehly, A. C. (2006) The Assay of Catalases and Peroxidases, in Methods of Biochemical Analysis, Volume 1 (ed D. Glick), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470110171.ch14
Sun W, Kadima TA, Pickard MA, Dunford HB (1994) Catalase activity of chloroperoxidase and its interaction with peroxidase activity. Biochemistry and Cell Biology 72:321- 331.
Dunford HB (1999) Heme Peroxidases. Wiley-VCH. New York, pp 435-453.
Schonrogge K, Harper LJ, Lichtenstein CP (2000) The protein content of tissue in cynipid galls (Hymenoptera: Cynipidae): similarities between cynipid galls and seeds. Plant Cell and Environment 23:215-222. http://onlinelibrary.wiley.com/doi/ 10.1046/j.1365-3040.2000.00543.x/pdf
de Oliveira DC, Isaias RMS (2010) Redifferentiation of leaflet tissues during gall midrib gall development in Copaifera langsdorffii (Fabaceae). South African Journal of Botany 76:239-248. doi:10.1016/j.sajb.2009.10.011
de Oliveira DC, Magalhaes TA, Carneiro RGS, Alvim MN, Isaias RMS (2010) Do Cecidomyiidae galls of Aspidosperma spruceanum (Apocynaceae) fit the pre-established cytological and histochemical patterns? Protoplasma 242: 81-93. doi:10.1007/s00709-010-0128-6
Shorthouse JD, Rohfritsch O (eds.) (1992). Biology of insectinduced galls. Oxford University Press, New York.
Anete Teixeira Formiga, Geraldo Luiz Gonçalves Soare, Rosy Mary dos Santos Isaias (2011) Responses of the Host Plant Tissues to Gall Induction in Aspidosperma spruceanum Müell. Arg. (Apocynaceae). American Journal of Plant Sciences, 2: 823-834. http://www.SciRP.org/journal/ajps
Chaman ME, Corcuera LJ, Zuniga GE, Cardemil L, Argandona VH (2001) Induction of soluble and cell wall peroxidises by aphid infestation in barley. Journal of Agriculture and Food Chemistry 49:2249-2253. http://www. ncbi.nlm.nih.gov/pubmed/11368584
Ni X, Quisenberry SS, Hegn-moss J, Markwell J, Sarath G, Klucas R, Baxendale F (2001) Oxidative responses of resistant and susceptible cereal leaves to symptomatic and nonsymptomatic cereal aphid (Hemiptera: Aphididae) feeding. Ecological Entomology 94:743-751. http://digitalcom-mons.unl.edu/cgi/viewcontent.cgi?article=1118&context=ento mologyfacpub
Isobe K, Inoue N, Takamatsu Y, Kamada K, Wakao N (2006) Production of catalase by fungi growing at low pH and high temperature. Journal of Bioscience and Bioengineering 101:73-76. https://www.jstage.jst.go.jp/article/jbb/101/1/101_ 1_73/_pdf
Felton GW, Donato K, Del Vecchio RJ, Duffey SS (1989) Activation of plant foliar oxidases by insect feeding reduces nutritive quality of foliage for noctuid herbivores. Journal of Chemical Ecology 15: 2667-2694. http://link.springer.com/ article/10.1007%2FBF01014725
Tscharntke T, Thiessen S, Dolch R, Boland W (2001) Herbivory, induced resistance and interplant signal transfer in Alnus glutinosa. Biochemical Systematics and Ecology 29:1025-1047. https://www.researchgate.net/publication/ 222704419_Herbivory_induced_resistance_and_interplant_si gnal_transfer_in_Alnus_glutinosa
Nurmi K, Ossipov V, Haukioja E, Pihlaja K (1996). Variation of total phenolic content and individual low-molecular-weight phenolics in foliage of mountain birch trees. Journal of Chemical Ecology 22: 2023-2040. http://link.springer.com/ article/10.1007/BF02040093
Felton GW, Duffey SS (1991). Protective action of midgut catalase in lepidopteran larvae against oxidative plant defences. Journal of Chemical Ecology 17:1715-1732. http://www.ncbi.nlm.nih.gov/pubmed/24257916
Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant and Cell Physiology 42: 462-468. http://pcp.oxfordjournals.org/ content/42/5/462.full.pdf+html
Ruuhola T, Yang S (2006) Wound – induced Oxidative Responses in Mountain Birch Leaves. Annals of Botany 97:29-37. http://aob.oxfordjournals.org/content/97/1/29.full. pdf
Gibon Y, Sulpice R, Larher F (2000) Proline accumulation in canola leaf discs subjected to osmotic stress is related to the loss of chlorophylls and to the decrease of mitochondrial activity. Physiologia Plantarum 110: 469-476. http://onlinelibrary.wiley.com/doi/10.1111/j.1399-3054.2000.1100407. x/abstrac
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Copyright (c) 2014 Suparna Mandal Biswas, Nabanita Chakraborty, Baidyanath Pal
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