Prevalence of Specific IgE to Dust and Storage Mites in Patients from Buildings with Moisture Damage Using Pharmacia- and DPC-Specific IgE Immunoglobulin Assays
DOI:
https://doi.org/10.12974/2312-5470.2018.05.2Keywords:
Storage mite, house dust mite, mite exposure, allergy tests, mouldy buildings.Abstract
The interpretation of the results of in vitro tests for allergy to different kinds of mites may be challenging for clinicians due to the methodological differences between commercial tests.
We assessed the presence of specific IgE-antibodies to house dust and storage mites in the serum of 24 patients with respiratory symptoms, and in 24 healthy controls, using two methods: the Pharmacia UniCAP System® (today Thermo Fisher Scientific. Allergy. Phadia AB) and DPC Alastat assays®. We also tested a storage mite mix reagent from Pharmacia. The presence and characterisation of the mites in the dust samples were assessed using microscopy. The Pharmacia experimental storage mite mix was positive for 11 out of 12 patients in a Pharmacia positive specific test. In the patient sera, Pharmacia detected 61 positive specific responses, whereas DPC detected 24. This indicates a significant analytical difference between the methods.
The presence of identifiable mites or mite allergens in dust found by microscopy might confirm the IgE response. Combining the specific IgE test for the house mite Dermatophagoides pteronyssinus and a storage mite mix test, including the mites Acarus siro, Glycophagus domesticus, Lepidoglyphus destructor and Tyrophagus putrescentiae, can produce a cost-effective estimate of a suspected mite sensitisation case (IgE response).
References
Dutkiewicz J, Jablonski L and Olenchock SA. Occupational Biohazards: A Rev Am J Ind Med 1988; 14: 605-623. https://doi.org/10.1002/ajim.4700140511
Fernandez-Caldas E, Puerta L and Caraballo L. Mites and allergy. Chem Immunol Allergy 2014; 100: 234-242. https://doi.org/10.1159/000358860
Ruggieri S, Drago G, Longo V, Columbo, et al. RESPIRA Project Group. Sensization to dust mite defines different phenotypes of asthma: A multicenter study. Pediatr Allergy Immunol 2017; 28: 675-682. https://doi.org/10.1111/pai.12768
Ghos A, Dutta S, Podder S, Mondal P, et al. Sensitivity to house dust mites allergens with atopic asthma and its relationship with CD14C(-159T) polymorphism in patients of West Bengal, India. J Med Entomol 2018; 55: 14-19. https://doi.org/10.1093/jme/tjx178
Pirilä V and Kilpiö O. Occupational mite dermatitis. Acta Dermato Venerology 1954; 34: 368-71.
Saleh AM, Ali HA, Ahmed SA, Mohammad NM and Morsy TA. House dust mites: a risk factor to be considered for occupational safety or source of work-related allergens. J Egypt Soc Parasitol 2013; 43: 669-678. https://doi.org/10.12816/0006423
Green WF and Woolcock AJ. Tyrophagus putrescentiae: an allergenically important mite. Clin Allergy 1978; 8: 135-144. https://doi.org/10.1111/j.1365-2222.1978.tb00458.x
Hertzen LV and Haahtela T. House dust mites in atopic diseases: accused for 45 years but not guilty? Am J Resp Crit Care Med 2009; 180: 113-119. https://doi.org/10.1164/rccm.200807-1001CO
Cuthbert OD. Storage mite allergy. Clin Rev Allergy 1990; 8: 69-86.
Iversen M, Korsgaard J, Hallas T and Dahl R. Mite allergy and exposure to storage mites and house dust mites in farmers. Clin Exp Allergy 1990; 29: 211-219. https://doi.org/10.1111/j.1365-2222.1990.tb02670.x
Lehtinen PT and Oksala I. Mite allergies in manufacturing and trade I. Grain storages, mills, feed factories and malt houses. Report for Finnish Work Environment Fund 1988, project 85194. (In Finnish).
Solarz K and Solarz D. The allergenic mites in coal-mine dust from coal mines in upper Silesia (Poland). Ann Agric Environ Med 1996; 3: 9-55.
Solarz K, Szilman P and Szilman E. Preliminary study on the occurrence and species composition of astigmatic mites (Acari: Astigmata) in samples of dust, debris and residues from farming environments in Poland. Ann Agric Environ Med 1997; 4: 249-252.
Solarz, K. The allergenic acarofauna of the house dust from dwellings, hospitals, libraries and institutes in upper Silesia (Poland): Ann Agric Environ Med 1998; 5: 73-85.
Terho EO, Leskinen L, Husman K and Kärelampi L. Occurrence of storage mites in Finnish farming environments. Allergy 1982; 37: 15-19. https://doi.org/10.1111/j.1398-9995.1982.tb04112.x
Van Hage-Halmsten M and Johansson E. Clinical and immunological aspects of storage mite allergy. Allergy 1998; 53(48 Suppl): 49-53. https://doi.org/10.1111/j.1398-9995.1998.tb04997.x
Pennanen S, Mussalo-Rauhamaa H, Harju A, Pippuri M, Liesivuori J, Elg P, Hakala K, Haahtela T. Exposure to mites, sensitisation and allergy to mites in moisture damaged buildings. Ind Built Environ 2007; 16: 19-27. https://doi.org/10.1177/1420326X06075036
Jaakkola MS, Ieromnimon A and Jaakkola JJ. Are atopy and spesific IgE to mites and molds important for adult asthma? J Allergy Clin Immunol 2006; 117: 642-648. https://doi.org/10.1016/j.jaci.2005.11.003
Garcia-Robaina JC, Eraso E, De la Torre F, Guisantes J, Martinez A, Palacios R, Martinez J. Extracts from various mite species contain cross-reactive and noncross-reactive IgE epitopes. A RAST inhibition study. J Investig Allergol Clin Immunol 1998; 8(5): 285-9.
Gafvelin G, Johansson E, Lundin A, Smith AM, Chapman MD, Benjamin DC, Derewenda U, van Hage-Hamsten M. Cross-reactivity studies of a new group 2 allergen from the dust mite Glycophagus domesticus, Gly d 2, and group 2 allergens from Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae with recombinant allergens. Allergy Clin Immunol 2001; 107(3): 511-8. https://doi.org/10.1067/mai.2001.112264
21. Boquete M, Carballada F, Armisen M, Nieto A, Martin S, Polo F, Carreira J. Factors influencing the clinical picture and the differential sensitization to house dust mites and storage mites. J Investig Allergol Clin Immunol 2000; 10(4): 229-34.
