Can Increased Endotoxin Levels in House Dust be used as an Indicator of a Mould Problem?
DOI:
https://doi.org/10.12974/2312-5470.2018.05.1Keywords:
Mould, pets, endotoxin, health.Abstract
Endotoxins are common indoor biocontaminants. Our purpose was to investigate the association of endotoxin content with mould problems in house dust in buildings, to determine whether endotoxins can be used as an indicator of the presence of moulds in buildings. The participants were selected from the Clinic for Indoor Air Health Problems on the basis of symptoms attributed to indoor air problems. We examined their health status. House dust was collected in new vacuum cleaner bags from surfaces in the rooms of their homes or workplaces. We measured the endotoxin concentrations in the house dust of 13 homes and 9 workplaces of the 19 participants, using a quantitative limulus test. The median endotoxin concentrations in the house dust of their homes was 9.9, and the range was 1.9–103 EU/mg; and in the house dust of workplaces it was 4.5, and the range 0.5–81 EU/mg. We found higher house dust endotoxin concentrations in rooms in which moisture problems had been identified or in dwellings in which pets were kept. Our pilot study suggests that endotoxins may be a useful additional tool for estimating possible mould contamination at homes or workplaces.
References
Zielen S, Trischler J and Schubert R. Lipopolysaccharide challenge: immunological effects and safety in human. Expert Rev Clin Immunol 2015; 11: 409-418. https://doi.org/10.1586/1744666X.2015.1012158
Rennie DC, Lawson JA, Senthilselvan A, Wilson PJ and Dosman JA. Domestic entoxin exposure and asthma in children: epidemiological studies. Front. Biosci. /Elite ED) 2012; 1: 56-73. https://doi.org/10.2741/e360
Duchaine C, Grimard Y and Cormier Y. Influence of building maintenance, environmental factors, and seasons on airborne contaminations of swine confinement buildings. AIHAJ 2000; 61: 56-63. https://doi.org/10.1202/0002- 8894(2000)061<0056:IOBMEF>2.0.CO;2
Hyvärinen A, Roponen M, Tiittanen P, Laitinen S, Nevalainen A and Pekkanen J. Dust sampling methods for endotoxin – an essential, but underestimated issue. Indoor Air 2006; 16: 20-27. https://doi.org/10.1111/j.1600-0668.2005.00392.x
Barnig C, Reboux G, Roussel S, Casset A, Sohy C, Dalphin JC, deBlay F. Indoor dust and air concentrations of endotoxin in urban and rural environments. Lett Appl Microbiol 2013; 56: 161-167. https://doi.org/10.1111/lam.12024
Douwes J, Doekes G, Heinrich J, Koch A, Bischof W and Brunekreef B. Endotoxin and (β1→3)-glucan in house dust and the relation with home characteristics: A pilot study in 25 German houses. Indoor Air 1998; 255-263. https://doi.org/10.1111/j.1600-0668.1998.00006.x
Venge P, Byström L, Håkansson L and Moberg L. Eosinophil cationic protein (ECP) is taken up by and stirred in neutrophil granulocytes. Allergy Clin Immunol. Int 1997; Suppl 4:116.
Mussalo-Rauhamaa H, Hakala K, Kiviranta K, Metso T and Haahtela T. Increased myeloperoxidase (MPO) in induced sputum in patients with a history of indoor air health problems. In: Proceedings of Healthy Buildings 2000, Exposure, Human Responses and Building Investigations, vol. 1., Eds. O. Seppänen, J. Säteri, SIY Indoor Air, Information Oy, Helsinki, Finland/ Gummerus Kirjapaino Oy, Jyväskylä, pp. 263- 266.
Heinrich J, Gehring U, Douvest J, Koch A, Fahlbusch B, et al. Pets and vermin are associated with high endotoxin levels in house dust. Clin Exp Allergy 2001; 31: 1839-1845. https://doi.org/10.1046/j.1365-2222.2001.01220.x
Larsson L, Szponar B, Pehrson C. Tobacco smoking increases dramatically air concentrations of endotoxin. Indoor Air 2004; 14: 421-424. https://doi.org/10.1111/j.1600-0668.2004.00290.x
Heinrich J, Holscher B, Douwes J, Richter K, Koch A, et al. INGA Study Group. Reproducibility of allergen, endotoxin and fungi measurements in the indoor environment. J Exp Anal Environ Epidemiol 2003; 13: 152-60. https://doi.org/10.1038/sj.jea.7500267
Koch A, Heilemann KJ and Bischof W. Storage stability of viable mould spores and endotoxin in house dust samples. Proceedings of the Indoor Air, Monterey, California 2002; 46- 51.
Bischof W, Kock A, Gehring U, Fahlbusch B and Heinrich J. Predictors of high endotoxin concentrations in the settled dust of German homes. Indoor Air 2002; 12: 2-9. https://doi.org/10.1034/j.1600-0668.2002.120102.x
Ownby DR, Peterson EL, Wegienka G, Woodecroft KJ, Nicholas C, et al. Are cats and dogs the major source of endotoxin in homes? Indoor Air 2013; 23: 219-226. https://doi.org/10.1111/ina.12016
Gehring U, Douwes J, Diekes G, Koch A, Bischof W, et al. INGA Study Group. Indoor Factors and Genetics in Asthma. Beta (1-3)-glucan in house dust of German homes: housing characteristics, occupant behaviour, and relations with endotoxins. Allergens and molds. Environ Health Perspect 2001; 109: 139-144.
Huang YJ, Huang JY, Lin LL, Wu PC, Ma YP and Su HJ. Environmental endotoxin concentraions are associated with home characteristics. In: Proceedings of the Indoor Air 2002, Monterey, California, p. 764-769.
Rylander R. Endotoxin in the environment - exposure and effects. J Endotoxin Res 2002; 8: 241-252. https://doi.org/10.1179/096805102125000452
Bornehag CG, Blomquist G, Gyntelberg F, Jarvholm B, Malmberg P, et al. Dampness in buildings and health. Nordic interdisciplinary review of the scientific evidence on association between exposure to 'dampness' in buildings and health effects. Indoor Air 2001; 11: 72-86. https://doi.org/10.1034/j.1600-0668.2001.110202.x
Mussalo-Rauhamaa H, Nikulin M, Koukila-Kähkölä P, Hintikka EL, Malmberg M and Haahtela T. Health effects of residents exposed to Stachybotrys in water-damaged houses in Finland. Indoor and Built Environment 2010; 19: 476-485. https://doi.org/10.1177/1420326X10367290
Mendell MJ, Mirer AG, Cheung K, Tong M and Douwes J. Respiratory and allergic health effects of dampness, mold, and dampness-related agents: a review of the epidemiologic evidence. Environ Health Perspect 2011; 119: 748-756. https://doi.org/10.1289/ehp.1002410
Michel O, Nagy AM, Schroven M, Duchateau J, Neve J, et al. Dose-response relationship to inhaled endotoxin in normal subjects. Am J Respir Crit Care Med 1997; 156: 1157-1164. https://doi.org/10.1164/ajrccm.156.4.97-02002
Von Mutius E and Radon K. Living on a farm: impact on asthma induction and clinical course. Immunol Allergy Clin North Am 2008; 28: 631-634. https://doi.org/10.1016/j.iac.2008.03.010
Mendy A, Gasana J, Vieira ER, Forno E, Patel J, et al. Endotoxin exposiure and childhood wheeze and asthma: a meta-analaysis of observational srudies. J Asthma 2011; 48: 685-693. https://doi.org/10.3109/02770903.2011.594140
Carlsten C, Ferguson A, Dimich-Ward H, Chan H, DyBuncio A, et al. Association between endotoxin and mite allergen exposure with asthma and specific sensitization at age 7 in high-risk children. Pediatr Allergy Immunol 2011; 22: 320- 325. https://doi.org/10.1111/j.1399-3038.2010.01123.x
Thischer C, Gehring U, Chen CM, Kerkhof M, Koppelman G, et al. Respiratory health in children , and indoor exposure to (1,3)- β--D-glucan, EPS mould components and endotoxin. Eur Respir J 2011; 37: 1050-1059. https://doi.org/10.1183/09031936.00091210
Lai PS, Sheehan WJ, Gaffin JM, Petty CR, Coull BA, et al. School endotoxin exposure and asthma morbidity in innercity children. Chest 2015; 148: 1251-1258. https://doi.org/10.1378/chest.15-0098
Jacobs JH, Krop EJ, de Wind S, Spithoven J and Heederik DJ. Endotoxin levels in homes and classrooms of Dutch school children and respiratory health. Eur Respir J 2013; 42: 314-322. https://doi.org/10.1183/09031936.00084612
Newton AN, Davis M, Koehler K, Shreffler W, Ahluwalia S, et al. Atopy as a modifier of the relationships between endotoxin exposure and symptoms among laboratory animal workers. Work Expo Health 2017; 61: 1024-1028. https://doi.org/10.1093/annweh/wxx061
Thorne PS, Mendy A, Metwali N, Salo P, Jaramillo R, et al. Endotoxin exposure: predictors and prevalence of associated asthma outcomes in the United States. Am J Respir Crit Care Med 2015; 1287-1297. https://doi.org/10.1164/rccm.201502-0251OC
Carnes MU, Hoppin JA, Metwali N, Wyss AB, Hankinson JL, et al. House dust endotoxin levels are associated with adult asthma in a U.S. Farming population. Ann Am Thorac Soc 2017; 14: 324-331. https://doi.org/10.1513/AnnalsATS.201611-861OC
Douwes J, Pearce N and Heederik D. Does environmental endotoxin exposure prevent asthma? Thorav 2002; 57: 86-90. https://doi.org/10.1136/thorax.57.1.86
World Health Organisation. WHO guidelines for indoor air quality: dampness and mould. WHO Regional Office for Europe 2009; 228 p.
